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東京大学 大学院総合文化研究科 広域科学専攻 生命環境科学系  Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo

公表Publication

誌上発表(査読あり)

51  Y. Nihongaki, Y. Furuhata, T. Otabe, S. Hasegawa, K. Yoshimoto and M. Sato, "CRISPR–Cas9-based photoactivatable transcription systems to induce neuronal differentiation",
Nature Methods, in press.
[DOI: 10.1038/nmeth.4430
50  K.i Hirose , M. Tsuchida , H. Asakura , K.i Wakui , K. Yoshimoto , K. Iida , M. Sato , M.i Shibukawa , M.i Suganuma and S. Saito, "A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis",
Analyst, in press.
[DOI: 10.1039/C7AN00909G
49  M. Komiyama, K. Yoshimoto, M. Sisido, and K. Ariga, "Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics",
Bulletin of the Chemical Society of Japan, 90 (9), 967-1004 (2017).
[DOI: 10.1246/bcsj.20170156Open Access
48  Furuhata, Y., Yoshitomi, T. Kikuchi, Y., Sakao, M., Yoshimoto, K., "Osteogenic Lineage Commitment of Adipose-Derived Stem Cells is Predetermined by Three-Dimensional Cell Accumulation on Micropatterned Surface",
ACS Applied Materials & Interfaces, 9 (11), 9339-9347 (2017).
[DOI: 10.1021/acsami.6b15688
47 冨田 峻介,吉本 敬太郎,丹羽 修,栗田 僚二 "交差反応的光学フィンガープリンティングを利用するタンパク質センシング",
分析化学, 66 (1), 1-10 (2017).
[DOI: 10.2116/bunsekikagaku.66.1] Open Access
46 Furuhata, Y., Kikuchi, Y., Tomita, S., Yoshimoto, K., "Small Spheroids of Adipose-Derived Stem Cells with Time-Dependent Enhancement of IL-8 and VEGF-A Secretion",
Genes to Cells, 21 (12), 1380-1386 (2016).
[DOI: 10.1111/gtc.12448]
45 Yoshioka, J., Yoshitomi, T., Yasukawa, T., Yoshimoto, K., "Alternation of Gene Expression Levels in Mesenchymal Stem Cells by Applying Positive Dielectrophoresis", 
Analytical Science, 32(11), 1213-1216 (2016).
[DOI: 10.2116/analsci.32.1213Open Access
44 Tomita, S., Yokoyama, S., Kurita, R., Niwa, O., Yoshimoto, K., "The use of an enzyme-based sensor array to fingerprint proteomic signatures of sera from different mammalian species", 
Analytical Science, 32(2), 237 (2016).
[DOI: 10.2116/analsci.32.237Open Access
43  Saito, S., Hirose, K., Tsuchida, M., Wakui, K., Yoshimoto, K., Nishiyama, Y., Shibukawa, M., "Rapid Acquisition of High-Affinity DNA Aptamer Motifs Recognizing Microbial Cell Surfaces Using Polymer-Enhanced Capillary Transient Isotachophoresis",
Chemical Communications, 52, 461-464 (2016).
[DOI: 10.1039/C5CC07268AOpen Access
42  Tomita, S., Sakao, M., Kurita, R., Osamu, N., Yoshimoto, K., "A Polyion Complex Sensor Array for Markerless and Noninvasive Identification of Differentiated Mesenchymal Stem Cells from Human Adipose Tissue", 
Chemical Science
, 6(10), 5831-5836 (2015).
[DOI: 10.1039/C5SC01259GOpen Access
41 Tomita, S., Soejima, T., Shiraki, K., Yoshimoto, K., "Enzymatic fingerprinting of structurally similar homologous proteins using polyion complex library constructed by tuning PEGylated polyamine functionalitiesion ", 
Analyst, 139(23), 6100-6103 (2014). [Link] Open Access
40 Tomita, S., Yoshimoto, K., "Polyion complex libraries possessing naturally occurring differentiation for pattern-based protein discrimination", 
Chemical Communications
, 49, 10430-10432 (2013). [Link] Open Access
39 Saito, S., Kawashima, M., Ohshima, H., Enomoto, K., Sato, M., Yoshimura, H., Yoshimoto, K.,Maeda, M., Shibukawa, M., "Separation of metalloproteins using a novel metal ion contaminant sweeping technique and detection of protein-bound copper by a metal ion probe in polyacrylamide gel electrophoresis: distribution of copper in human serum", 
Analyst
, 138, 6097-6105 (2013). [Link] Open Access
38 Yuan, X., Fabregat, D., Yoshimoto, K., Nagasaki, Y., "Development of a high-performance immunolatex based on "soft landing" antibody immobilization mechanism", 
Colloids and Surfaces B: Biointerfaces
, 99, 45-52 (2012). [Link] 
37 Ikeda, Y., Jomura, T., Horiuchi, U., Saeki, J., Yoshimoto, K., Ikeya, T., Nagasaki, Y., "Long-term survival and functional maintenance of hepatocytes by using a microfabricated cellarray",  
Colloids and Surfaces B: Biointerfaces
, 97, 97-100 (2012). [Link]
36 Yuan, X., Fabregat, D., Yoshimoto, K., Nagasaki, Y. "High PEGylation Efficiency of Pentaethylenehexamine-end Poly(ethyleneglycol) (mPEG-N6) for Active-ester Surface", 
Colloids and Surfaces B: Biointerfaces
, 92,25-29 (2012).[Link]
35 Saito, S., Nakano, Y., Hikichi, A., Suzuki, R., Yoshimoto, K., Maeda, M., Aoyama, M., Shibukawa, M., "Ultrasensitive CE for heavy metal ions using the variations in the chemical structures formed from new octadentate fluorescent probes and cationic polymers" 
Analyst
, 136(13),2697-2705 (2011). [Link]
34 Kubota, M., Yoshimoto, K., Yuan, X., Nagasaki, Y., "Improvement of the thermal stability of streptavidin immobilized on magnetic beads by the construction of a mixed-poly(ethylene glycol) tethered-chain Layer" 
Polymer Journal
, 43, 493-496 (2011).[Link]
33 Yoshimoto, K., Nishio, M., Sugasawa, H., and Nagasaki, Y., "Direct Observation of Adsorption-Induced Inactivation of Antibody Fragments Surrounded by Mixed-PEG Layer on a Gold Surface",
Journal of the American Chemical Society, 132(23), 7982-7989 (2010). [Link]
32 Ganguli, S., Yoshimoto, K., Tomita, S., Sakuma, H., Matsuoka, T., Shiraki, K., and Nagasaki, Y., "Improving the Heat Resistance of Ribonuclease A by the Addition of Poly(N,N-diethylaminoethyl methacrylate)-graft-poly(ethylene glycol) (PEAMA-g-PEG)"
Macromolecular Bioscience, 10, 853-859 (2010). [Link]



Prior Publications
31 Yuan, X., Fabregat, D., Yoshimoto, K., Nagasaki, Y., "Efficient Inhibition of Interfacial Nonspecific Interaction to Create Practically Utilizable High Ferritin-Response Immunolatex"Analytical Chemistry, 81(24), 10097-10105 (2009). [Link]
30 Yoshimoto, K., Hirase, T., Madsen, J., Armes S. P., and Nagasaki, Y., "Non-Fouling Character of Poly[2-(methacryloyloxy)ethyl Phosphorylcholine] Modified Gold Surfaces Fabricated by the “Grafting to” Method: Comparison of its Protein Resistance with Poly(ethylene glycol) Modified Gold Surfaces"Macromolecular Rapid Communication, 30(24), 2136-2140 (2009). [Link].
29 Yoshimoto, K., Nozawa, M., Matsumoto, S., Echigo, T., Nemoto, S., Hatta, T., and Nagasaki, Y., "Studies on the Adsorption Property and Structure of Polyamine-Ended Poly(ethylene glycol) Derivatives on a Gold Surface by Surface Plasmon Resonance and Angle-resolved X-ray Photoelectron Spectroscopy" Langmuir, 25(20), 12243-12249 (2009). [Link]
28 Kojima, R., Yoshimoto, K., Miyoshi, H., and Nagasaki, Y., "Spheroid array of fetal mouse liver cells constructed on a PEG-gel micropatterned surface: upregulation of hepatic functions by co-culture with nonparenchymal liver cells" Lab on a Chip, 9, 1991-1993 (2009). [Link]
27 Ganguli, S., Yoshimoto, K., Tomita, S., Sakuma, H., Matsuoka, T., Shiraki, K., and Nagasaki, Y., "Regulation of Lysozyme Activity Based on Thermotolerant Protein/Smart Polymer Complex Formation"Journal of the American Chemical Society, 131 (18), 6549-6553 (2009).[Link]
26 Atsumi, H., Yoshimoto, K., Saito, S., Ohkuma, M., Maeda, M., and Nagasaki Y., "Luminescence-based Colorimetric Discrimination of Single-nucleotide Transversions by the Combined Use of the Derivatives of DOTA-conjugated Naphthyridine and Its Terbium Complex" Tetrahedron Letters, 50, 2177-2180 (2009).[Link]
25 Yoshimoto, K., Ichino, M., and Nagasaki, Y., "Inverted Pattern Formation of Cell Microarrays on Poly(ethylene glycol) (PEG) Gel Patterned Surface and Construction of Hepatocyte Spheroids on Unmodified PEG Gel Microdomains" Lab on a Chip, 9, 1286-1289 (2009).[Link]
24 Sato, Y., Nishizawa, S., Yoshimoto, K., Seino, T., Morita, K., Teramae N., "Influence of substituent modifications on the binding of 2-amino-1,8-naphthyridines to cytosine opposite an AP site in DNA duplexes: Thermodynamic characterization" Nucleic Acid Research, 37(5), 1411-1422 (2009).[Link]
23 Yuan, X., Yoshimoto, K., Nagasaki Y., "High-performance Immunolatex Possessing A Mixed-PEG/Antibody Coimmobilized Surface: Highly Sensitive Ferritin Immunodiagnostics" Analytical Chemistry, 81(4), 1549-1556 (2009).[Link]
22 Yoshimoto, K., Hoshino, Y., Ishii, T., Nagasaki Y., "Binding Enhancement of Antigen-Functionalized PEGylated Gold Nanoparticles onto Antibody-Immobilized Surface by Increasing the Functionalized Antigen using α-sulfanyl-ω-amino-PEG" Chemical Communications, 5369-5371 (2008).[Link]
21 Yoshimoto, K., Hirase, T., Nemoto, S., Hatta, T., Nagasaki, Y., "Facile Construction of Sulfanyl-Terminated Poly(ethylene glycol)-Brushed Layer on a Gold Surface for Protein Immobilization by the Combined Use of Sulfanyl-Ended Telechelic and Semitelechelic Poly(ethylene glycol)s" Langmuir 24 (17), 9623-9629 (2008).[Link]
20 PSoga, K., Tsuji, T., Tashiro, F., Chiba, J., Oishi, M., Yoshimoto, K., Nagasaki, Y., Kitano, K., Hamaguchi, S., "Development of NIR bioimaging systems" Journal of Physics: Conference Series 106, No pp given (2008).
19 PNagasaki, Y., Ichino, M., Yoshimoto, K., "Biosurface Design for Patterned Cell Culture Engineering"Transactions of the Materials Research Society of Japan 33 (3), 755-758 (2008).
18 Miyamoto, D., Oishi, M., Kojima, K., Yoshimoto, K., Nagasaki, Y., "Completely Dispersible PEGylated Gold Nanoparticles under Physiological Conditions: Modification of Gold Nanoparticles with Precisely Controlled PEG-b-polyamine" Langmuir 24 (9), 5010-5017 (2008).
17 Yoshimoto, K., Matsumoto, S., Asakawa, R., Uchida, K., Ishii, T., Nagasaki, Y., "Immobilization and hybridization behavior of DNA on poly(ethylene glycol)-block-poly[2-(N,N-dimethylamino)ethyl methacrylate]-modified gold surfaces" Chem. Lett. 36 (12), 1444-1445 (2007).
16 Uchida, K., Hoshino, Y., Tamura, A., Yoshimoto, K., Kojima, S., Yamashita, K., Yamanaka, I., Otsuka, H., Kataoka, K., Nagasaki, Y., "Creation of a mixed poly(ethylene glycol) tethered-chain surface for preventing the nonspecific adsorption of proteins and peptides" Biointerphases 2 (4), 126-130 (2007).
15 Saito, S., Takeuchi, S., Yoshimoto, K., Maeda, M., Aoyama, M., "Direct fluorescence detection of ultratrace lanthanide(III) ions complexed with aromatic polyaminocarboxylate, avoiding quenching of ligand-centered emission, using capillary zone electrophoresis with a ternary complexing technique"Analyst 132 (3), 237-241 (2007).
14 Saito, S., Suzuki, R., Danzaka, N., Hikichi, A., Yoshimoto, K., Maeda, M., Aoyama, M., "Direct fluorometric detection of paramagnetic and heavy metal ions at sub-amol level using an aromatic polyaminocarboxylate by CZE: combination of pre- and on-capillary complexation technique"Electrophoresis 28 (14), 2448-2457 (2007).
13 Saito, S., Shimidzu, J.-I., Yoshimoto, K., Maeda, M., Aoyama, M., "Selective ultratrace detection of Al(III) and Ga(III) complexed with a calcein isomer by capillary zone electrophoresis with laser-induced fluorescence detection" J. Chromatogr., A 1140 (1-2), 230-235 (2007).
12 Sankaran, N.B., Nishizawa, S., Seino, T., Yoshimoto, K., Teramae, N., "Abasic-site-containing oligodeoxynucleotides as aptamers for riboflavin" Angew. Chem., Int. Ed. 45 (10), 1563-1568 (2006).
11 Nishizawa, S., Sankaran, N.B., Seino, T., Cui, Y.-Y., Dai, Q., Xu, C.-Y., Yoshimoto, K., Teramae, N., "Use of vitamin B2 for fluorescence detection of thymidine-related single-nucleotide polymorphisms"Anal. Chim. Acta 556 (1), 133-139 (2006).
10 PHoshino, Y., Kimura, R., Chiba, J., Yoshimoto, K., Nagasaki, Y., "Construction of His6-Protein A/PEG Co-immobilized Surface for High-Performance Protein Sensing" Transactions of the Materials Research Society of Japan 32 (2), 571-574 (2006).
09 Dai, Q., Xu, C.-Y., Sato, Y., Yoshimoto, K., Nishizawa, S., Teramae, N., "Enhancement of the binding ability of a ligand for nucleobase recognition by introducing a methyl group" Anal. Sci. 22 (2), 201-203 (2006).
08 西澤 精一,  吉本 敬太郎 , 清野 丈博, 許 春燕, 寺前 紀夫, ''水素結合性小分子による核酸塩基認識と一塩基多型蛍光検出'', 分析化学 53 (5), 383-391 (2004).
07 Nishizawa, S., Yoshimoto, K., Seino, T., Xu, C.-Y., Minagawa, M., Satake, H., Tong, A., Teramae, N., "Fluorescence detection of cytosine/guanine transversion based on a hydrogen bond forming ligand" Talanta 63 (1), 175-179 (2004).
06 Yoshimoto, K., Xu, C.-Y., Nishizawa, S., Haga, T., Satake, H., Teramae, N., "Fluorescence detection of guanine-adenine transition by a hydrogen bond forming small compound" Chem. Commun.(24), 2960-2961 (2003).
05 Yoshimoto, K., Nishizawa, S., Minagawa, M., Teramae, N., "Use of abasic site-containing DNA strands for nucleobase recognition in water" J. Am. Chem. Soc. 125 (30), 8982-8983 (2003).
04 Nishizawa, S., Yokobori, T., Kato, R., Yoshimoto, K., Kamaishi, T., Teramae, N., "Hydrogen-bond forming ionophore for highly efficient transport of phosphate anions across the nitrobenzene-water interface" Analyst 128 (6), 663-669 (2003).
03 吉本 敬太郎, 金子 恵美子, 四ッ柳 隆夫, ''ブロモクロロフェノールブルーとメンブランフィルターを用いる尿中タンパク質の簡易目視定量'', 分析化学, 49 (6), 363-367 (2000).
02 Yoshimoto, K., Kaneko, E., Yotsuyanagi, T., "Color change of ternary associate by drying on membrane filter for visual protein detection" Chem. Lett. (1), 6-7 (2000).
01 Kaneko, E., Yoshimoto, K., Yotsuyanagi, T., "Visual drop test of hemoglobin in whole blood by using ring-like concentration of rhodamine B onto octadecylsilanized silica plate" Chem. Lett. (8), 751-752 (1999).
 

 P: 学会関連雑誌


誌上発表(査読なし)

04 Yoshimoto, K., Kojima, R., Takahashi, E., Ichino, M., Miyoshi, H., Nagasaki, Y., "3D Cell Co-culture System on Hydrogel Micro-Patterned Surface Fabricated by Photolithography", Journal of Photopolymer Science and Technology, 25, 1, 45-52 (2012).[PDF]
03 Yoshimoto, K., Atsumi, H., Saito, S., Okuma, M., Maeda, M., Nagasaki, Y., "Fluorescence-based affinity labeling of nucleobase by hydrogen-bond forming metal complex" Nucleic Acids Symp. Ser.(51), 303-304 (2007).
02 Takarada, T., Imaizumi, E., Kino, K., Yoshimoto, K., Ogasawara, S., Kanayama, N., Maeda, M., "Detection of base pairing of an oxidatively damaged guanine using colloidal stability change of DNA-linked polymer micelles" Nucleic Acids Symp. Ser. (51), 305-306 (2007).
01 Yoshimoto, K., Nishizawa, S., Koshino, H., Sato, Y., Teramae, N., Maeda, M., "Assignment of hydrogen-bond structure in a ligand-nucleobase complex inside duplex DNA: combined use of quantum chemical calculations and 15N NMR experiments" Nucleic Acids Symp. Ser. (49), 255-256 (2005).
 

著書・総説・解説

09
金野 智浩, 吉本 敬太郎, ''4.2 バイオミメティック界面'', バイオマテリアルの基礎 (石原 一彦, 塙 隆夫, 前田 瑞夫 編), 142-147(2010).
08
Xiaofei Yuan, Dolca Fabregat, Keitaro Yoshimoto, Yukio Nagasaki, Biomaterials (ACS Symposium Series, Vol. 1054), Chapter 13, pp 243-258. Publication Date (Web): November 18, (2010).  
07
吉本 敬太郎, 長崎 幸夫, ''Grafting to法によるポリエチレングリコール密生層の構築と新しいバイオセンシング用インターフェースとしての利用'', バイオマテリアル-生体材料 27(3),180-188 (2009).
06
吉本 敬太郎, 星野 裕樹, 長崎 幸夫, ''ヘテロ二官能性ポリエチレングリコールによる機能性金ナノ粒子の創製'', 78(7), 644-649 (2008).
05
吉本 敬太郎, (共著:古性 均, 長崎 幸夫), ''ナノ粒子センサー'', ナノバイオ計測の実際  講談社サイエンティフィック(三原 久和, 小畠 英理, 馬場 嘉信監修)(分担執筆), (2007).
04
吉本 敬太郎, 古性 均, 長崎 幸夫, ''ナノ粒子センサーの発展'', 未来材料 7(6), 46-54 (2007).
03
吉本 敬太郎, (共著:西澤 精一, 寺前 紀夫), ''SNPs解析法-基礎と原理, 最新のSNPs typing chemistry'', 先端の分析法-理工学からナノ・バイオまで, エヌ・ティー・エス(梅澤 喜夫, 澤田 嗣郎, 寺部 茂 編)(分担執筆), (2004).
02
西澤 精一, 吉本 敬太郎, 寺前 紀夫, ''DNA脱塩基部位を利用する核酸塩基認識と一塩基変異蛍光検出'', 日本化学会生体機能関連部会 News Letter, 18(2), 6-9 (2003).
01
吉本 敬太郎, ''非ワトソン-クリック塩基対部位を認識する小分子プローブの設計'', 化学と工業 55(5), 582 (2002).


その他


1.特許

07
発明者:吉本 敬太郎, 古性 均, 稲見 有希, 山中 美弥子, 出願人:㈱日産化学工業, "ヒト大腸癌細胞Colo205に特異的に結合する核酸", 特願2014-146632, 出願日 2014/7/17.
06
 
 発明者:吉本 敬太郎, 古性 均, 稲見 有希, 出願人:㈱日産化学工業, "がん細胞に結合するDNAアプタマー", 特願2013-98230, 出願日 2013/5/8.
05
発明者:長崎 幸夫, 原 暁非, 吉本 敬太郎, 出願人:筑波大学, "混合ポリエチレングリコールと抗体が共固定化された表面を所有する免疫ラテックス粒子及びその製造方法", 特願2009-7846.
04
発明者:長崎 幸夫, 小島 綾太, 吉本 敬太郎出願人:筑波大学, "胎生肝細胞のスフェロイドを含む培養細胞構築法"特願2008-328057. 
03
発明者:寺前 紀夫, 西澤 精一, 吉本 敬太郎, 清野 丈博, 佐藤 冬樹出願人:Japan Science and Technology Agency, Japan, "Detection of SNP using probes and fluorescent receptor"国際特許WO2005090604.
02
発明者:西澤 精一, 寺前 紀夫, 吉本 敬太郎出願人:東北大学および東京インスツルメント(㈱), "一塩基置換検出法及び一塩基置換検出用キット", JP 2004261068.
01
発明者:吉本 敬太郎,子 恵美子, 四ツ柳 隆夫, 出願人:株式会社シノテスト(㈱), "試料中のタンパク質の分析方法及 びそのキット", JP 11304803.  

 


2.依頼講演
13
Yoshimoto, K., "Upregulation of Cellar Function Using 3D Cell Culture Microenvironment Constructed by Adhesion-Controlled Micro-Patterned Surface'', International Workshop on Soft Interface Sciences for Young Scientists 2012(SISYS2012)(22nd ,November 2012, AIST, Tsukuba, Japan) . 
12
Yoshimoto, K., Nagasaki, Y., "Effect of 3D Cell Culture Environment Using Hydrogel Micro-Patterned Surface on Cell Differentiation'', International Union of Materials Research Societies(IUMRS)(27th, September 2012, PACIFICO-YOKOHAMA, Kanagawa, Japan).
11
Yoshimoto, K., "3D Cell Co-culture System on Hydrogel Micro-Patterned Surface Fabricated by Photolithography'', International Conference of Photopolymer Science and Technology (ICPST)(27th, June 2012, University of Chiba).
10
吉本 敬太郎, "高分子修飾表面の界面力‐生体高分子・細胞に与える不思議な環境‐ "表面力セミナー (2012年3月3日,東北大学) .
09
Yoshimoto, K., "3D Cell Co-culture System on Hydrogel Micro-Patterned Surface'', MRS Fall Meeting(29th, December 2011, Hynes Convention Centerm Boston MA., USA).
08
吉本 敬太郎, ''培養細胞の構造制御と高機能化-細胞も3Dの時代!?'', 12回機能構造と分析化学シンポジウム(2011年11月19日, 東北大学).
07
吉本 敬太郎, ''分子認識の選択性を高めるソフト界面設計'', 日本分析化学会第60年会, (2011年9月14日, 名古屋大学).
06
吉本 敬太郎, "生体高分子の機能を向上させる天然/合成高分子ハイブリッド型密生層界面 " 
日本化学会第90春季年会 特別企画講演、33-ソフト界面科学のニューウェーブ(2010年3月26日,近畿大学).
05
Yoshimoto, K., "Function of Poly(ethylene glycol) Modified Layers Prepared by "Grafting to" Method on Gold Surface", 3rd International Symposium on Interdisciplinary Materials Science for Biomaterials Science, (3rd ISIMSBMS-2009)(24th, April 2009, University of Tsukuba).
04
吉本 敬太郎, "Grafting to 法により構築される PEG 誘導体修飾表面の不思議 " 表面技術協会ナノテク部会第28回研究会 (2009130日,東京理科大学).
03
吉本 敬太郎, "生体高分子/合成高分子共密生層界面を有するインテリジェントセンシングデバイスの創製 "6回ライフサーベイヤーシンポジウム 2008626日,大阪大学).
02
吉本 敬太郎, "核酸の脱塩基部位形成を利用する一塩基変異蛍光診断法 "20回緑陰セミナー・美瑛(2005619-20日,北海道・美瑛,国立大雪少年の家).
01 吉本 敬太郎, "核酸の脱塩基部位形成を利用する分子認識法の開発と一塩基多型蛍光検出 "2003年度日本分析化学会東北支部奨励賞受賞講演(20043月,東北大学).

 



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