招待講演・受賞情報はこちら Invites / Awards
吉本 敬太郎, 坂田 飛鳥, 稲見 有希 “サイボーグRNAアプタマー創薬を天然型DNAアプタマーでrebootする”（情報からマテリアルへ : ノンコーディングRNA研究 : 機能分子としてのRNAを見つけ、知り、創薬に使う新時代 | 第3章 RNAを “使う” ―RNAを使い細胞を操作する）
実験医学増刊, 42 (15), 154-162 (2024). [PDF]
吉冨 徹, 吉本 敬太郎*, “血管内皮増殖因子（VEGF）受容体を認識する核酸アプタマー”（総合論文・特集：バイオ分析の新潮流）
分析化学, 73 (3), 71-78 (2024).
[DOI: bunsekikagaku.73.71]
Muneyuki Matsuo, Koji Wakui, Yuuki Inami, Akihiro Furukawa, Seiji Sato, Keitaro Yoshimoto*, “Proficiently partitioning of bioactive peptide-ssDNA conjugates by microbead-assisted capillary electrophoresis (MACE)”
Analytical Biochemistry, 687, 115452 (2024).
[DOI: 10.1016/j.ab.2023.115452]
Masanobu Nagano, Kazuki Kubota, Asuka Sakata, Rei Nakamura, Toru Yoshitomi, Koji Wakui, Keitaro Yoshimoto*, “A neutralizable dimeric anti-thrombin aptamer with potent anticoagulant activity in mice”
Molecular Therapy – Nucleic Acids, 33, 762-772 (2023).
[DOI: 10.1016/j.omtn.2023.07.038]
➡ 解説：EurekAlert! 08/29, AlphaGalileo 08/29
➡ 解説：東京大学 PRESS RELEASES (0808 Japanese/ 08/29 English)
➡ 解説：東京大学 大学院総合文化研究科・ニュース 2023 08/08
Romualdo Troisi, Valeria Napolitano, Emanuele Rossitto, Waleed Osman, Masanobu Nagano, Koji Wakui, Grzegorz M. Popowicz, Keitaro Yoshimoto, and Filomena Sica*, “Steric hindrance and structural flexibility shape the functional properties of a guanine-rich oligonucleotide”
Nucleic Acids Research, 51 (16), 8880-8890 (2023).
[DOI: 10.1093/nar/gkad634]
PDB: 8BW5 (Thrombin-M08s-1_41mer aptamer complex)
➡ 解説：東京大学 PRESS RELEASES 2023 08/01
➡ 解説：東京大学 大学院総合文化研究科・ニュース 2023 08/02
Nagano M., Toda T., Makino K., Miki H., Sugizaki Y., Tomizawa H., Isobayashi A., Yoshimoto K.*, “Discovery of a highly specific anti-methotrexate (MTX) DNA aptamer for antibody-independent MTX detection”
Analytical Chemistry, 94 (49), 17255–17262 (2022).
[DOI: 10.1021/acs.analchem.2c04182]
➡ 解説：東京大学 大学院総合文化研究科・ニュース 2023 01/23
Nagano M., Nakano S., Yoshimoto K.*, “Evaluation of G-quartet-forming deoxyguanines in antiparallel G-quadruplexes using optical spectroscopy and deoxyguanine-to-deoxythymidine scanning”
Analytical Biochemistry, 658, 114903 (2022).
[DOI: 10.1016/j.ab.2022.114903]
Nagano M., Oguro T., Sawada R., Yoshitomi T., Yoshimoto K.*, “Accelerated Discovery of Potent Bioactive anti-TNFα Aptamers by Microbead-Assisted Capillary Electrophoresis (MACE)-SELEX”
ChemBioChem, 22, 3341-3347 (2021).
[DOI: 10.1002/cbic.202100478]
Yoshitomi T.*, Karita H., Mori-Moriyama N., Sato N., Yoshimoto K.*, “Reduced cytotoxicity of polyethyleneimine by covalent modification of antioxidant and its application to microalgal transformation”
Science and Technology of Advanced Materials, 22(1), 864-874 (2021).
[DOI: 10.1080/14686996.2021.1978273]
Saito S.*, Sakamoto T., Tanaka N., Watanabe R., Kamimura T., Ota K., Riley K. R., Yoshimoto K., Tasaki-Handa Y., Shibukawa M., “Single-Round DNA Aptamer Selection by Combined Use of Capillary Electrophoresis and Next Generation Sequencing: An Aptaomics Approach for Identifying Unique Functional Protein-Binding DNA Aptamers”
Chemistry A European Journal, 27, 10058-1067 (2021).
[DOI: 10.1002/chem.202100177]
Yoshitomi T., Wakui K., Miyakawa M., Yoshimoto K.*, “Design Strategy of Short Antidote Sequence for Bivalent aptamer: Rapid Neutralization of High Anticoagulant Thrombin-Binding DNA Aptamer Linked M08 with HD22”
Research and Practice in Thrombosis and Haemostasis, 5(5), e12503, 1-7 (2021).
[DOI: 10.1002/rth2.12503] 分子認識化学・核酸化学・薬学（抗血液凝固）
Yoshitomi, T.*, Zheng, H., Yoshimoto, K.*, “Investigations of chirality effects on undifferentiated state of mesenchymal stem cells using soft nanofibrous oligopeptide hydrogels”
Analytical Sciences, 37(3), 539-543 (2021).
[DOI: 10.2116/analsci.20SCN05]
Yoshitomi T., Hayashi M., Oguro T., Kimura K., Wayama F., Furusho H., Yoshimoto, K.*, “Binding and structural properties of DNA aptamers with VEGF-A-mimic activity”
Molecular Therapy Nucleic Acids, 19, 1145-1152 (2020).
[DOI: 10.1016/j.omtn.2019.12.034] 分子認識化学・核酸化学・細胞工学（血管新生）
Yoshitomi T.*, Kaminaga S., Sato N., Toyoshima M., Moriyama T., Yoshimoto K.*, “Formation of Spherical Palmelloid Colony with Enhanced Lipid Accumulation by Gel Encapsulation of Chlamydomonas debaryana NIES-2212”
Plant and Cell Physiology, 61(1), 158-168 (2020).
[DOI: 10.10.1093/pcp/pcz188]
Yoshitomi T.*, Shimada N., Iijima K., Hashizume M., Yoshimoto K., “Polyethyleneimine-induced astaxanthin accumulation in the green alga Haematococcus pluvialis by increased oxidative stress”
Journal of Bioscience and Bioengineering, 128(6), 751-754 (2019).
[DOI: 10.1016/j.jbiosc.2019.06.002] 高分子化学・植物学（藻類・ｶﾙﾃｲﾉｲﾄﾞ生産）
Yasukawa T.*, Morishima A., Suzuki M., Yoshioka J., Yoshimoto K., Mizutani F., “Rapid Formation of Aggregates with Uniform Numbers of Cells Based on a Three-dimensional Dielectrophoresis”
Analytical Sciences, 35(8), 895-901 (2019).
[DOI: 10.2116/analsci.19P074] 分析化学（分離）・誘電泳動・細胞工学（3D 培養）
Selected as Hot Articles | go to Volume 35(8), 2019.
Wakui, K., Abe, A., Yoshitomi, T., Furusho, H., Yoshimoto, K.*, “High Enrichment of Nucleobase-Modified Aptamers in Early Selection Rounds by Microbeads-Assisted Capillary Electrophoresis SELEX”
Analytical Sciences, 35(5), 585-588 (2019).
[DOI: 10.2116/analsci.18SDN04] 分析化学（分離）・分子認識化学・核酸化学
Selected as Hot Articles | go to Volume 35(5), 2019.
Wakui, K., Yoshitomi, T., Yamaguchi, A., Tsuchida, M., Saito, S., Shibukawa, M., Furusho, H., Yoshimoto, K.*, “Rapidly Neutralizable and High Anticoagulant Thrombin-Binding DNA Aptamer Discovered by Microbeads-Assisted Capillary Electrophoresis (MACE) SELEX”
Molecular Therapy Nucleic Acids, 16, 348-359 (2019).F = 8.886]
[DOI: 10.1016/j.omtn.2019.03.002] 分析化学（分離）・分子認識化学・核酸化学・薬学（抗血液凝固）
Furuhata, Y.*, Kobayashi, M., Maruyama, R., Sato, Y., Makino, K., Michiue, T., Yui, H., Nishizawa, S., Yoshimoto, K.*, “Programmable RNA Detection with a Fluorescent RNA Aptamer Using Optimized Three-way Junction Formation”
RNA, 25, 590-599 (2019).
[DOI: 10.1261/rna.069062.118] 分析化学（計測・プローブ）・分子認識化学・核酸化学
Yoshitomi, T., Wayama, F., Kimura, K., Wakui, K., Furusho, H., Yoshimoto, K.*, “Screening of DNA signaling aptamer from multiple candidates obtained from SELEX with next-generation sequencing”
Analytical Sciences, 35(1), 113 (2019).
[DOI: 10.2116/analsci.18SDN05] 分析化学（計測・プローブ）・分子認識化学・核酸化学
Book
Miura Y. and Yoshimoto K., “Chapter 3. Nano- and Micro-technology of Soft Interface”
Molecular Soft-Interface Science (Springer Nature), 55-74 (2019).
[DOI: 10.1007/978-4-431-56877-3_3]
[ISBN: 978-4-431-56875-9 (Print), 978-4-431-56877-3 (Online)]
Highlight (mini review)
Yoshimoto K., “Selection Technologies and Applications of Nucleic Acid Aptamers”
Analytical Sciences, 35(10), 1063-1064 (2019).
[DOI: 10.2116/analsci.highlights1910]
Stem Cell Engineering
Yoshioka, J., Ohsugi, Y., Yoshitomi, T., Yasukawa, T., Sasaki, N., Yoshimoto, K.*, “Label-free rapid separation and enrichment of bone marrow-derived mesenchymal stem cells from heterogeneous cell mixture using a dielectrophoresis device”
Sensors, 18(9), 3007 (8 pages) (2018).
[DOI: 10.3390/s18093007]
Stem Cell Engineering
Zheng, H., Yoshitomi, T., Yoshimoto, K.*, “Analysis of Chirality Effects on Stem Cell Fate Using Three-dimensional Fibrous Peptide Hydrogels”
ACS Applied Bio Materials, 1 (3), 538-543 (2018).
[DOI: 10.1021/acsabm.8b00123]
Bio Analytical Method
Maruyama, R., Makino, K., Yoshitomi, T., Yui, H., Furusho, H., Yoshimoto, K.*, “Estimation of G-Quartet-Forming Guanines in Parallel-Type G-Quadruplexes by Optical Spectroscopy Measurements of Their Single-Nucleobase Substitution Sequences”
Analyst, 143, 4022-4026 (2018).
[DOI: 10.1039/C8AN01131A]
Bio Analytical Method
Tomita, S.*, Nomoto, H., Yoshitomi, T., Iijima, K., Hashizume, M., Yoshimoto, K.*, “Noninvasive fingerprinting-based tracking of replicative cellular senescence using a colorimetric Polyion Complex Array“
Analytical Chemistry, 90(11), 6348-6352 (2018).
[DOI: 10.1021/acs.analchem.8b00795] 解説
Biomaterials
Iwasaki, Y.*, Yokota, A., Otaka, A., Inoue, N., Yamaguchi, A., Yoshitomi, T., Yoshimoto, K., Neo, M., “Bone-targeting poly(ethylene sodium phosphate)”,
Biomaterials Science, 6, 91-95 (2018).
[DOI: 10.1039/C7BM00930E]
Stem Cell Engineering
Furuhata, Y., Nihongaki, Y., Sato, M., Yoshimoto, K.*, “Control of adipogenic differentiation in mesenchymal stem cells via endogenous gene activation using CRISPR-Cas9”,
ACS Synthetic Biology, 6 (12), 2191–2197 (2017).
[DOI: 10.1021/acssynbio.7b00246] 解説
Aptamer, Bio Analytical Method
Hirose, K., Tsuchida, M., Asakura, H., Wakui, K., Yoshimoto, K., Iida, K., Sato, M., Shibukawa, M., Suganuma, M., and Saito, S.*, “A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis”,
Analyst, 142 (21), 4030-4038 (2017).
[DOI: 10.1039/C7AN00909G]
Stem Cell Engineering
Nihongaki, Y., Furuhata, Y., Otabe, T., Hasegawa, S., Yoshimoto, K., and Sato, M.*, “CRISPR–Cas9-based photoactivatable transcription systems to induce neuronal differentiation”,
Nature Methods, 14, 963-966 (2017).
[DOI: 10.1038/nmeth.4430] 解説
Stem Cell Engineering
Furuhata, Y., Yoshitomi, T., Kikuchi, Y., Sakao, M., Yoshimoto, K.*, “Osteogenic Lineage Commitment of Adipose-Derived Stem Cells is Predetermined by Three-Dimensional Cell Accumulation on Micropatterned Surface”,
ACS Applied Materials & Interfaces, 9 (11), 9339-9347 (2017).
[DOI: 10.1021/acsami.6b15688] 解説
Review
Komiyama, M., Yoshimoto, K., Sisido, M., and Ariga, K., “Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics”,
Bulletin of the Chemical Society of Japan, 90 (9), 967-1004 (2017).
[DOI: 10.1246/bcsj.20170156] Open Access
Review
吉本 敬太郎 “間葉系幹細胞を外側から制御・診断するポリマーエンジニアリング”
FBC Lett. (生命科学研究レター), 54, 10-15 (2017).
[PDF]
Review
冨田 峻介, 吉本 敬太郎, 丹羽 修, 栗田 僚二 “交差反応的光学フィンガープリンティングを利用するタンパク質センシング”,
分析化学, 66 (1), 1-10 (2017).
[DOI: 10.2116/bunsekikagaku.66.1] Open Access
Stem Cell Engineering
Furuhata, Y., Kikuchi, Y., Tomita, S., Yoshimoto, K.*, “Small Spheroids of Adipose-Derived Stem Cells with Time-Dependent Enhancement of IL-8 and VEGF-A Secretion”,
Genes to Cells, 21 (12), 1380-1386 (2016).
[DOI: 10.1111/gtc.12448]
Micro Device, Stem Cell Engineering
Yoshioka, J., Yoshitomi, T., Yasukawa, T., Yoshimoto, K.*, “Alternation of Gene Expression Levels in Mesenchymal Stem Cells by Applying Positive Dielectrophoresis”,
Analytical Sciences, 32(11), 1213-1216 (2016).
[DOI: 10.2116/analsci.32.1213] Open Access
Selected as a Journal Cover | go to Cover of Volume 32(11), 2016.
Bio Analytical Method
Tomita, S., Yokoyama, S., Kurita, R., Niwa, O., Yoshimoto, K., “The use of an enzyme-based sensor array to fingerprint proteomic signatures of sera from different mammalian species”,
Analytical Sciences, 32(2), 237-240 (2016).
[DOI: 10.2116/analsci.32.237] Open Access
Aptamer, Bio Analytical Method
Saito, S.*, Hirose, K., Tsuchida, M., Wakui, K., Yoshimoto, K., Nishiyama, Y., Shibukawa, M., “Rapid Acquisition of High-Affinity DNA Aptamer Motifs Recognizing Microbial Cell Surfaces Using Polymer-Enhanced Capillary Transient Isotachophoresis”,
Chemical Communications, 52, 461-464 (2016).
[DOI: 10.1039/C5CC07268A] Open Access
Review
冨田 峻介, 吉本 敬太郎 “セクレトームフィンガーパタン分析を実現するバイオマテリアル設計”
バイオマテリアル―生体材料―, 34, 46-49 (2016)
[PDF] Open using “tomita” as password
Bio Analytical Method, Stem Cell
Tomita, S.*, Sakao, M., Kurita, R., Osamu, N., Yoshimoto, K.*, “A Polyion Complex Sensor Array for Markerless and Noninvasive Identification of Differentiated Mesenchymal Stem Cells from Human Adipose Tissue”,
Chemical Science, 6(10), 5831-5836 (2015).
[DOI: 10.1039/C5SC01259G] Open Access
Bio Analytical Method
Tomita, S.*, Soejima, T., Shiraki, K., Yoshimoto, K.*, “Enzymatic fingerprinting of structurally similar homologous proteins using polyion complex library constructed by tuning PEGylated polyamine functionalitiesion “,
Analyst, 139(23), 6100-6103 (2014).
[DOI: 10.1039/C4AN01398K] Open Access
Bio Analytical Method
Tomita, S.*, Yoshimoto, K.*, “Polyion complex libraries possessing naturally occurring differentiation for pattern-based protein discrimination”,
Chemical Communications, 49, 10430-10432 (2013).
[DOI: 10.1039/C3CC45640D] Open Access
Bio Analytical Method
Saito, S.*, Kawashima, M., Ohshima, H., Enomoto, K., Sato, M., Yoshimura, H.,Yoshimoto, K.,Maeda, M., Shibukawa, M., “Separation of metalloproteins using a novel metal ion contaminant sweeping technique and detection of protein-bound copper by a metal ion probe in polyacrylamide gel electrophoresis: distribution of copper in human serum”,
Analyst, 138, 6097-6105 (2013).
[DOI: 10.1039/C3AN01107K] Open Access
Review
冨田 峻介, 吉本 敬太郎 “交差反応型センサアレイを用いるクルードなタンパク質溶液の評価”
生物工学会誌, 93, 285-288 (2015).
[PDF]
Yuan, X., Fabregat, D., Yoshimoto, K., Nagasaki, Y.*, “Development of a high-performance immunolatex based on “soft landing” antibody immobilization mechanism”,
Colloids and Surfaces B: Biointerfaces, 99, 45-52 (2012).
Ikeda, Y., Jomura, T., Horiuchi, U., Saeki, J., Yoshimoto, K., Ikeya, T., Nagasaki, Y.*, “Long-term survival and functional maintenance of hepatocytes by using a microfabricated cellarray”,
Colloids and Surfaces B: Biointerfaces, 97, 97-100 (2012).
Yuan, X., Fabregat, D., Yoshimoto, K., Nagasaki, Y*. “High PEGylation Efficiency of Pentaethylenehexamine-end Poly(ethyleneglycol) (mPEG-N6) for Active-ester Surface”,
Colloids and Surfaces B: Biointerfaces, 92,25-29 (2012).
Saito, S.*, Nakano, Y., Hikichi, A., Suzuki, R., Yoshimoto, K., Maeda, M., Aoyama, M., Shibukawa, M., “Ultrasensitive CE for heavy metal ions using the variations in the chemical structures formed from new octadentate fluorescent probes and cationic polymers”
Analyst, 136(13),2697-2705 (2011).
Kubota, M., Yoshimoto, K., Yuan, X., Nagasaki, Y.*, “Improvement of the thermal stability of streptavidin immobilized on magnetic beads by the construction of a mixed-poly(ethylene glycol) tethered-chain Layer”
Polymer Journal, 43, 493-496 (2011).
Yoshimoto, K., Nishio, M., Sugasawa, H., and Nagasaki, Y.*, “Direct Observation of Adsorption-Induced Inactivation of Antibody Fragments Surrounded by Mixed-PEG Layer on a Gold Surface”,
Journal of the American Chemical Society, 132(23), 7982-7989 (2010).
Ganguli, S., Yoshimoto, K., Tomita, S., Sakuma, H., Matsuoka, T., Shiraki, K., and Nagasaki, Y.*, “Improving the Heat Resistance of Ribonuclease A by the Addition of Poly(N,N-diethylaminoethyl methacrylate)-graft-poly(ethylene glycol) (PEAMA-g-PEG)”,
Macromolecular Bioscience, 10, 853-859 (2010).