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Furuhata Y., Kobayashi M., Makino K., Aoki K., Yui H., Yoshimoto K., submitted.
Yoshitomi T., Kimura K., Hayashi M., Wayama F., Furusho F., Yoshimoto K., submitted.
Yoshitomi T., Kaminaga S., Sato N., Toyoshima M., Moriyama T., Yoshimoto K., submitted.

2019 and Accepted

Toru Yoshitomi, Naoya Shimada, Kazutoshi Iijima, Mineo Hashizume, Keitaro Yoshimoto, “Polyethyleneimine-induced astaxanthin accumulation in the green alga Haematococcus pluvialis by increased oxidative stress”
Journal of Bioscience and Bioengineering, accepted.

Yasukawa T., Morishima A., Suzuki M., Yoshioka J., Yoshimoto K., Mizutani F., “Rapid Formation of Aggregates with Uniform Numbers of Cells Based on a Three-dimensional Dielectrophoresis”
Analytical Sciences, in press.
[DOI: 10.2116/analsci.19P074]

Wakui, K., Abe, A., Yoshitomi, T., Furusho, H., Yoshimoto, K., “High Enrichment of Nucleobase-Modified Aptamers in Early Selection Rounds by Microbeads-Assisted Capillary Electrophoresis SELEX”
Analytical Sciences, 35(5), 585 (2019).
[DOI: 10.2116/analsci.18SDN04]
Selected as Hot Articles | go to Volume 35(5), 2019.

Wakui, K., Yoshitomi, T., Yamaguchi, A., Tsuchida, M., Saito, S., Shibukawa, M., Furusho, H., Yoshimoto, K., “Rapidly Neutralizable and High Anticoagulant Thrombin-Binding DNA Aptamer Discovered by Microbeads-Assisted Capillary Electrophoresis (MACE) SELEX”
Molecular Therapy Nucleic Acids, 16, 348-359 (2019).
[DOI: 10.1016/j.omtn.2019.03.002]

Furuhata, Y., Kobayashi, M., Maruyama, R., Sato, Y., Makino, K., Michiue, T., Yui, H., Nishizawa, S., Yoshimoto, K., “Programmable RNA Detection with a Fluorescent RNA Aptamer Using Optimized Three-way Junction Formation”
RNA, 25, 590-599 (2019).
[DOI: 10.1261/rna.069062.118]

Yoshitomi, T., Wayama, F., Kimura, K., Wakui, K., Furusho, H.,  Yoshimoto, K., “Screening of DNA signaling aptamer from multiple candidates obtained from SELEX with next-generation sequencing”
Analytical Sciences, 35(1), 113 (2019).
[DOI: 10.2116/analsci.18SDN05]


Miura Yioshiko and Keitaro Yoshimoto, “Chapter 3. Nano- and Micro-technology of Soft Interface”, Molecular Soft-Interface Science (Springer Nature), 55-74 (2019).
[DOI: 10.1007/978-4-431-56877-3_3]
[ISBN: 978-4-431-56875-9 (Print), 978-4-431-56877-3 (Online)]


Yoshioka, J., Ohsugi, Y., Yoshitomi, T., Yasukawa, T., Sasaki, N., Yoshimoto, K., “Label-free rapid separation and enrichment of bone marrow-derived mesenchymal stem cells from heterogeneous cell mixture using a dielectrophoresis device”
Sensors, 18(9), 3007 (8 pages) (2018).
[DOI: 10.3390/s18093007]

Zheng, H., Yoshitomi, T.,  Yoshimoto, K.,  “Analysis of Chirality Effects on Stem Cell Fate Using Three-dimensional Fibrous Peptide Hydrogels”
ACS Applied Bio Materials, 1 (3), 538-543 (2018).
[DOI: 10.1021/acsabm.8b00123]

Maruyama, R.,  Makino, K.,  Yoshitomi, T.,  Yui, H.,  Furusho, H.,  Yoshimoto, K., “Estimation of G-Quartet-Forming Guanines in Parallel-Type G-Quadruplexes by Optical Spectroscopy Measurements of Their Single-Nucleobase Substitution Sequences”
Analyst, 143, 4022-4026 (2018).
[DOI: 10.1039/C8AN01131A]

Tomita, S.,  Nomoto, H.,  Yoshitomi, T.,  Iijima, K.,  Hashizume, M., Yoshimoto, K.,  “Noninvasive fingerprinting-based tracking of replicative cellular senescence using a colorimetric Polyion Complex Array“
Analytical Chemistry, 90(11), 6348-6352 (2018).
[DOI: 10.1021/acs.analchem.8b00795解説

Iwasaki, Y.,  Yokota, A.,  Otaka, A.,  Inoue, N.,  Yamaguchi, A.,  Yoshitomi, T.,  Yoshimoto, K.,  Neo, M., “Bone-targeting poly(ethylene sodium phosphate)”,
Biomaterials Science, 6, 91-95 (2018).
[DOI: 10.1039/C7BM00930E]


Furuhata, Y.,  Nihongaki, Y.,  Sato, M.,  Yoshimoto, K.,  “Control of adipogenic differentiation in mesenchymal stem cells via endogenous gene activation using CRISPR-Cas9”,
ACS Synthetic Biology, 6 (12), 2191–2197 (2017).
[DOI: 10.1021/acssynbio.7b00246]  解説

Hirose, K.,  Tsuchida, M.,  Asakura, H.,  Wakui, K.,  Yoshimoto, K.,  Iida, K.,  Sato, M.,  Shibukawa, M.,  Suganuma, M.,  and Saito, S., “A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis”,
Analyst, 142 (21), 4030-4038 (2017).
[DOI: 10.1039/C7AN00909G]

Nihongaki, Y.,  Furuhata, Y.,  Otabe, T.,  Hasegawa, S.,  Yoshimoto, K.,  and Sato, M.,  “CRISPR–Cas9-based photoactivatable transcription systems to induce neuronal differentiation”,
Nature Methods, 14, 963-966 (2017).
[DOI: 10.1038/nmeth.4430]  解説

Furuhata, Y.,  Yoshitomi, T.,  Kikuchi, Y.,  Sakao, M.,  Yoshimoto, K., “Osteogenic Lineage Commitment of Adipose-Derived Stem Cells is Predetermined by Three-Dimensional Cell Accumulation on Micropatterned Surface”,
ACS Applied Materials & Interfaces, 9 (11), 9339-9347 (2017).
[DOI: 10.1021/acsami.6b15688]  解説


Komiyama, M.,  Yoshimoto, K.,  Sisido, M.,  and Ariga, K.,  “Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics”,
Bulletin of the Chemical Society of Japan, 90 (9), 967-1004 (2017).
[DOI: 10.1246/bcsj.20170156]  Open Access

吉本 敬太郎 “間葉系幹細胞を外側から制御・診断するポリマーエンジニアリング”
FBC Lett. (生命科学研究レター), 54, 10-15 (2017).

冨田 峻介, 吉本 敬太郎, 丹羽 修, 栗田 僚二 “交差反応的光学フィンガープリンティングを利用するタンパク質センシング”,
分析化学, 66 (1), 1-10 (2017).
[DOI: 10.2116/bunsekikagaku.66.1] Open Access


Furuhata, Y., Kikuchi, Y., Tomita, S., Yoshimoto, K., “Small Spheroids of Adipose-Derived Stem Cells with Time-Dependent Enhancement of IL-8 and VEGF-A Secretion”,
Genes to Cells, 21 (12), 1380-1386 (2016).
[DOI: 10.1111/gtc.12448]

Yoshioka, J., Yoshitomi, T., Yasukawa, T., Yoshimoto, K., “Alternation of Gene Expression Levels in Mesenchymal Stem Cells by Applying Positive Dielectrophoresis”,
Analytical Sciences, 32(11), 1213-1216 (2016).
[DOI: 10.2116/analsci.32.1213] Open Access
Selected as a Journal Cover | go to Cover of Volume 32(11), 2016.

Tomita, S., Yokoyama, S., Kurita, R., Niwa, O., Yoshimoto, K., “The use of an enzyme-based sensor array to fingerprint proteomic signatures of sera from different mammalian species”,
Analytical Sciences, 32(2), 237-240 (2016).
[DOI: 10.2116/analsci.32.237] Open Access

Saito, S., Hirose, K., Tsuchida, M., Wakui, K., Yoshimoto, K., Nishiyama, Y., Shibukawa, M., “Rapid Acquisition of High-Affinity DNA Aptamer Motifs Recognizing Microbial Cell Surfaces Using Polymer-Enhanced Capillary Transient Isotachophoresis”,
Chemical Communications, 52, 461-464 (2016).
[DOI: 10.1039/C5CC07268A] Open Access


冨田 峻介, 吉本 敬太郎 “セクレトームフィンガーパタン分析を実現するバイオマテリアル設計”
バイオマテリアル―生体材料―, 34, 46-49 (2016)
[PDF] Open using “tomita” as password


Tomita, S., Sakao, M., Kurita, R., Osamu, N., Yoshimoto, K., “A Polyion Complex Sensor Array for Markerless and Noninvasive Identification of Differentiated Mesenchymal Stem Cells from Human Adipose Tissue”,
Chemical Science, 6(10), 5831-5836 (2015).
[DOI: 10.1039/C5SC01259G] Open Access

Tomita, S., Soejima, T., Shiraki, K., Yoshimoto, K., “Enzymatic fingerprinting of structurally similar homologous proteins using polyion complex library constructed by tuning PEGylated polyamine functionalitiesion “,
Analyst, 139(23), 6100-6103 (2014).
[DOI: 10.1039/C4AN01398K] Open Access

Tomita, S., Yoshimoto, K., “Polyion complex libraries possessing naturally occurring differentiation for pattern-based protein discrimination”,
Chemical Communications, 49, 10430-10432 (2013).
[DOI: 10.1039/C3CC45640D] Open Access

Saito, S., Kawashima, M., Ohshima, H., Enomoto, K., Sato, M., Yoshimura, H.,Yoshimoto, K.,Maeda, M., Shibukawa, M., “Separation of metalloproteins using a novel metal ion contaminant sweeping technique and detection of protein-bound copper by a metal ion probe in polyacrylamide gel electrophoresis: distribution of copper in human serum”,
Analyst, 138, 6097-6105 (2013).
[DOI: 10.1039/C3AN01107K] Open Access


冨田 峻介, 吉本 敬太郎 “交差反応型センサアレイを用いるクルードなタンパク質溶液の評価”
生物工学会誌, 93, 285-288 (2015).


Yuan, X., Fabregat, D., Yoshimoto, K., Nagasaki, Y., “Development of a high-performance immunolatex based on “soft landing” antibody immobilization mechanism”,
Colloids and Surfaces B: Biointerfaces, 99, 45-52 (2012).

Ikeda, Y., Jomura, T., Horiuchi, U., Saeki, J., Yoshimoto, K., Ikeya, T., Nagasaki, Y., “Long-term survival and functional maintenance of hepatocytes by using a microfabricated cellarray”,
Colloids and Surfaces B: Biointerfaces, 97, 97-100 (2012).

Yuan, X., Fabregat, D., Yoshimoto, K., Nagasaki, Y. “High PEGylation Efficiency of Pentaethylenehexamine-end Poly(ethyleneglycol) (mPEG-N6) for Active-ester Surface”,
Colloids and Surfaces B: Biointerfaces, 92,25-29 (2012).

Saito, S., Nakano, Y., Hikichi, A., Suzuki, R., Yoshimoto, K., Maeda, M., Aoyama, M., Shibukawa, M., “Ultrasensitive CE for heavy metal ions using the variations in the chemical structures formed from new octadentate fluorescent probes and cationic polymers”
Analyst, 136(13),2697-2705 (2011).

Kubota, M., Yoshimoto, K., Yuan, X., Nagasaki, Y., “Improvement of the thermal stability of streptavidin immobilized on magnetic beads by the construction of a mixed-poly(ethylene glycol) tethered-chain Layer”
Polymer Journal, 43, 493-496 (2011).

Yoshimoto, K., Nishio, M., Sugasawa, H., and Nagasaki, Y., “Direct Observation of Adsorption-Induced Inactivation of Antibody Fragments Surrounded by Mixed-PEG Layer on a Gold Surface”,
Journal of the American Chemical Society, 132(23), 7982-7989 (2010).

Ganguli, S., Yoshimoto, K., Tomita, S., Sakuma, H., Matsuoka, T., Shiraki, K., and Nagasaki, Y., “Improving the Heat Resistance of Ribonuclease A by the Addition of Poly(N,N-diethylaminoethyl methacrylate)-graft-poly(ethylene glycol) (PEAMA-g-PEG)”,
Macromolecular Bioscience, 10, 853-859 (2010).