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Publication

招待講演・受賞情報はこちら Invites / Awards

2021

(1) Nagano M., Oguro T., Sawada R., Yoshitomi T., Yoshimoto K.*, “Accelerated Discovery of Potent Bioactive anti-TNFα Aptamers by Microbead-Assisted Capillary Electrophoresis (MACE)-SELEX”
ChemBioChem, in press.
[DOI: 10.1002/cbic.202100478] 分析化学(分離)・分子認識化学・核酸化学・薬学(PPI, 抗炎症作用) 

(2) Yoshitomi T.*, Karita H., Mori-Moriyama N., Sato N., Yoshimoto K.*, “Reduced cytotoxicity of polyethyleneimine by covalent modification of antioxidant and its application to microalgal transformation”
Science and Technology of Advanced Materials, in press. [IF = 8.090]
[DOI: 10.1080/14686996.2021.1978273] 高分子化学・植物学(藻類・形質転換)

(3) Saito S.*, Sakamoto T., Tanaka N., Watanabe R., Kamimura T., Ota K., Riley K. R., Yoshimoto K., Tasaki-Handa Y., Shibukawa M., “Single-Round DNA Aptamer Selection by Combined Use of Capillary Electrophoresis and Next Generation Sequencing: An Aptaomics Approach for Identifying Unique Functional Protein-Binding DNA Aptamers”
Chemistry A European Journal, 27, 10058-1067 (2021). [IF = 5.236]
[DOI: 10.1002/chem.202100177] 分析化学(分離)・分子認識化学・核酸化学

 

(4) Yoshitomi T., Wakui K., Miyakawa M., Yoshimoto K.*, “Design Strategy of Short Antidote Sequence for Bivalent aptamer: Rapid Neutralization of High Anticoagulant Thrombin-Binding DNA Aptamer Linked M08 with HD22”
Research and Practice in Thrombosis and Haemostasis, 5(5), e12503 (2021).
[DOI: 10.1002/rth2.12503] 分子認識化学・核酸化学・薬学(抗血液凝固)

 

(5) Yoshitomi, T.*, Zheng, H., Yoshimoto, K.*, “Investigations of chirality effects on undifferentiated state of mesenchymal stem cells using soft nanofibrous oligopeptide hydrogels”
Analytical Sciences, 37(3), 539-543 (2021). [IF = 2.081]
[DOI: 10.2116/analsci.20SCN05] 高分子化学・細胞工学(幹細胞分化)・ペプチド/光学異性体

2020

分子認識化学・核酸化学・細胞工学(血管新生)
(1) Yoshitomi T., Hayashi M., Oguro T., Kimura K., Wayama F., Furusho H., Yoshimoto, K.*, “Binding and structural properties of DNA aptamers with VEGF-A-mimic activity”
Molecular Therapy Nucleic Acids, 19, 1145-1152 (2020). [IF = 8.886]
[DOI: 10.1016/j.omtn.2019.12.034] 分子認識化学・核酸化学・細胞工学(血管新生)(2) Yoshitomi T.*, Kaminaga S., Sato N., Toyoshima M., Moriyama T., Yoshimoto K.*, “Formation of Spherical Palmelloid Colony with Enhanced Lipid Accumulation by Gel Encapsulation of Chlamydomonas debaryana NIES-2212”
Plant and Cell Physiology, 61(1), 158-168 (2020). [IF = 4.927]
[DOI: 10.10.1093/pcp/pcz188] 高分子化学・植物学(藻類・脂質生産)

 

2019

高分子化学・植物学(藻類・カルテノイド生産)
(1) Yoshitomi T.*, Shimada N., Iijima K., Hashizume M., Yoshimoto K., “Polyethyleneimine-induced astaxanthin accumulation in the green alga Haematococcus pluvialis by increased oxidative stress”
Journal of Bioscience and Bioengineering, 128(6), 751-754 (2019).
[DOI: 10.1016/j.jbiosc.2019.06.002] 高分子化学・植物学(藻類・カルテイノイド生産)

(2) Yasukawa T.*, Morishima A., Suzuki M., Yoshioka J., Yoshimoto K., Mizutani F., “Rapid Formation of Aggregates with Uniform Numbers of Cells Based on a Three-dimensional Dielectrophoresis”
Analytical Sciences, 35(8), 895-901 (2019).
[DOI: 10.2116/analsci.19P074] 分析化学(分離)・誘電泳動・細胞工学(3D 培養)
Selected as Hot Articles | go to Volume 35(8), 2019.
(3) Wakui, K., Abe, A., Yoshitomi, T., Furusho, H., Yoshimoto, K.*, “High Enrichment of Nucleobase-Modified Aptamers in Early Selection Rounds by Microbeads-Assisted Capillary Electrophoresis SELEX”
Analytical Sciences, 35(5), 585 (2019).
[DOI: 10.2116/analsci.18SDN04] 分析化学(分離)・分子認識化学・核酸化学
Selected as Hot Articles | go to Volume 35(5), 2019.
(4) Wakui, K., Yoshitomi, T., Yamaguchi, A., Tsuchida, M., Saito, S., Shibukawa, M., Furusho, H., Yoshimoto, K.*, “Rapidly Neutralizable and High Anticoagulant Thrombin-Binding DNA Aptamer Discovered by Microbeads-Assisted Capillary Electrophoresis (MACE) SELEX”
Molecular Therapy Nucleic Acids, 16, 348-359 (2019). [IF = 8.886]
[DOI: 10.1016/j.omtn.2019.03.002] 分析化学(分離)・分子認識化学・核酸化学・薬学(抗血液凝固) (5) Furuhata, Y.*, Kobayashi, M., Maruyama, R., Sato, Y., Makino, K., Michiue, T., Yui, H., Nishizawa, S., Yoshimoto, K.*, “Programmable RNA Detection with a Fluorescent RNA Aptamer Using Optimized Three-way Junction Formation”
RNA, 25, 590-599 (2019).
[DOI: 10.1261/rna.069062.118] 分析化学(計測・プローブ)・分子認識化学・核酸化学(6) Yoshitomi, T., Wayama, F., Kimura, K., Wakui, K., Furusho, H.,  Yoshimoto, K.*, “Screening of DNA signaling aptamer from multiple candidates obtained from SELEX with next-generation sequencing”
Analytical Sciences, 35(1), 113 (2019).
[DOI: 10.2116/analsci.18SDN05] 分析化学(計測・プローブ)・分子認識化学・核酸化学

 

Book
Miura Y. and Yoshimoto K., “Chapter 3. Nano- and Micro-technology of Soft Interface”
Molecular Soft-Interface Science (Springer Nature), 55-74 (2019).
[DOI: 10.1007/978-4-431-56877-3_3]
[ISBN: 978-4-431-56875-9 (Print), 978-4-431-56877-3 (Online)]

Highlight (mini review)
Yoshimoto K., “Selection Technologies and Applications of Nucleic Acid Aptamers”
Analytical Sciences, 35(10), 1063-1064 (2019).
[DOI: 10.2116/analsci.highlights1910]

2018

Stem Cell Engineering
(1) Yoshioka, J., Ohsugi, Y., Yoshitomi, T., Yasukawa, T., Sasaki, N., Yoshimoto, K.*, “Label-free rapid separation and enrichment of bone marrow-derived mesenchymal stem cells from heterogeneous cell mixture using a dielectrophoresis device”
Sensors, 18(9), 3007 (8 pages) (2018).
[DOI: 10.3390/s18093007]

Stem Cell Engineering
(2) Zheng, H., Yoshitomi, T.,  Yoshimoto, K.*,  “Analysis of Chirality Effects on Stem Cell Fate Using Three-dimensional Fibrous Peptide Hydrogels”
ACS Applied Bio Materials, 1 (3), 538-543 (2018).
[DOI: 10.1021/acsabm.8b00123]

Bio Analytical Method
(3) Maruyama, R.,  Makino, K.,  Yoshitomi, T.,  Yui, H.,  Furusho, H.,  Yoshimoto, K.*, “Estimation of G-Quartet-Forming Guanines in Parallel-Type G-Quadruplexes by Optical Spectroscopy Measurements of Their Single-Nucleobase Substitution Sequences”
Analyst, 143, 4022-4026 (2018). [IF = 4.616]
[DOI: 10.1039/C8AN01131A]

Bio Analytical Method
(4) Tomita, S.*,  Nomoto, H.,  Yoshitomi, T.,  Iijima, K.,  Hashizume, M., Yoshimoto, K.*,  “Noninvasive fingerprinting-based tracking of replicative cellular senescence using a colorimetric Polyion Complex Array“
Analytical Chemistry, 90(11), 6348-6352 (2018).
[DOI: 10.1021/acs.analchem.8b00795解説

Biomaterials
(5) Iwasaki, Y.*,  Yokota, A.,  Otaka, A.,  Inoue, N.,  Yamaguchi, A.,  Yoshitomi, T.,  Yoshimoto, K.,  Neo, M., “Bone-targeting poly(ethylene sodium phosphate)”,
Biomaterials Science, 6, 91-95 (2018).
[DOI: 10.1039/C7BM00930E]

2017

Stem Cell Engineering
(1) Furuhata, Y.,  Nihongaki, Y.,  Sato, M.,  Yoshimoto, K.*,  “Control of adipogenic differentiation in mesenchymal stem cells via endogenous gene activation using CRISPR-Cas9”,
ACS Synthetic Biology, 6 (12), 2191–2197 (2017).
[DOI: 10.1021/acssynbio.7b00246]  解説

Aptamer, Bio Analytical Method
(2) Hirose, K.,  Tsuchida, M.,  Asakura, H.,  Wakui, K.,  Yoshimoto, K.,  Iida, K.,  Sato, M.,  Shibukawa, M.,  Suganuma, M.,  and Saito, S.*, “A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis”,
Analyst, 142 (21), 4030-4038 (2017).
[DOI: 10.1039/C7AN00909G]

Stem Cell Engineering
(3) Nihongaki, Y.,  Furuhata, Y.,  Otabe, T.,  Hasegawa, S.,  Yoshimoto, K.,  and Sato, M.*,  “CRISPR–Cas9-based photoactivatable transcription systems to induce neuronal differentiation”,
Nature Methods, 14, 963-966 (2017).
[DOI: 10.1038/nmeth.4430]  解説

Stem Cell Engineering
(4) Furuhata, Y.,  Yoshitomi, T.,  Kikuchi, Y.,  Sakao, M.,  Yoshimoto, K.*, “Osteogenic Lineage Commitment of Adipose-Derived Stem Cells is Predetermined by Three-Dimensional Cell Accumulation on Micropatterned Surface”,
ACS Applied Materials & Interfaces, 9 (11), 9339-9347 (2017).
[DOI: 10.1021/acsami.6b15688]  解説

Review

Komiyama, M.,  Yoshimoto, K.,  Sisido, M.,  and Ariga, K.,  “Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics”,
Bulletin of the Chemical Society of Japan, 90 (9), 967-1004 (2017).
[DOI: 10.1246/bcsj.20170156]  Open Access

吉本 敬太郎 “間葉系幹細胞を外側から制御・診断するポリマーエンジニアリング”
FBC Lett. (生命科学研究レター), 54, 10-15 (2017).
[PDF]

冨田 峻介, 吉本 敬太郎, 丹羽 修, 栗田 僚二 “交差反応的光学フィンガープリンティングを利用するタンパク質センシング”,
分析化学, 66 (1), 1-10 (2017).
[DOI: 10.2116/bunsekikagaku.66.1] Open Access

2016

Stem Cell Engineering
(1) Furuhata, Y., Kikuchi, Y., Tomita, S., Yoshimoto, K.*, “Small Spheroids of Adipose-Derived Stem Cells with Time-Dependent Enhancement of IL-8 and VEGF-A Secretion”,
Genes to Cells, 21 (12), 1380-1386 (2016).
[DOI: 10.1111/gtc.12448]

Micro Device, Stem Cell Engineering
(2) Yoshioka, J., Yoshitomi, T., Yasukawa, T., Yoshimoto, K.*, “Alternation of Gene Expression Levels in Mesenchymal Stem Cells by Applying Positive Dielectrophoresis”,
Analytical Sciences, 32(11), 1213-1216 (2016).
[DOI: 10.2116/analsci.32.1213] Open Access
Selected as a Journal Cover | go to Cover of Volume 32(11), 2016.

Bio Analytical Method
(3) Tomita, S., Yokoyama, S., Kurita, R., Niwa, O., Yoshimoto, K., “The use of an enzyme-based sensor array to fingerprint proteomic signatures of sera from different mammalian species”,
Analytical Sciences, 32(2), 237-240 (2016).
[DOI: 10.2116/analsci.32.237] Open Access

Aptamer, Bio Analytical Method
(4) Saito, S.*, Hirose, K., Tsuchida, M., Wakui, K., Yoshimoto, K., Nishiyama, Y., Shibukawa, M., “Rapid Acquisition of High-Affinity DNA Aptamer Motifs Recognizing Microbial Cell Surfaces Using Polymer-Enhanced Capillary Transient Isotachophoresis”,
Chemical Communications, 52, 461-464 (2016).
[DOI: 10.1039/C5CC07268A] Open Access

Review

冨田 峻介, 吉本 敬太郎 “セクレトームフィンガーパタン分析を実現するバイオマテリアル設計”
バイオマテリアル―生体材料―, 34, 46-49 (2016)
[PDF] Open using “tomita” as password

2013-2015

Bio Analytical Method, Stem Cell
Tomita, S.*, Sakao, M., Kurita, R., Osamu, N., Yoshimoto, K.*, “A Polyion Complex Sensor Array for Markerless and Noninvasive Identification of Differentiated Mesenchymal Stem Cells from Human Adipose Tissue”,
Chemical Science, 6(10), 5831-5836 (2015).
[DOI: 10.1039/C5SC01259G] Open Access

Bio Analytical Method
Tomita, S.*, Soejima, T., Shiraki, K., Yoshimoto, K.*, “Enzymatic fingerprinting of structurally similar homologous proteins using polyion complex library constructed by tuning PEGylated polyamine functionalitiesion “,
Analyst, 139(23), 6100-6103 (2014).
[DOI: 10.1039/C4AN01398K] Open Access

Bio Analytical Method
Tomita, S.*, Yoshimoto, K.*, “Polyion complex libraries possessing naturally occurring differentiation for pattern-based protein discrimination”,
Chemical Communications, 49, 10430-10432 (2013).
[DOI: 10.1039/C3CC45640D] Open Access

Bio Analytical Method
Saito, S.*, Kawashima, M., Ohshima, H., Enomoto, K., Sato, M., Yoshimura, H.,Yoshimoto, K.,Maeda, M., Shibukawa, M., “Separation of metalloproteins using a novel metal ion contaminant sweeping technique and detection of protein-bound copper by a metal ion probe in polyacrylamide gel electrophoresis: distribution of copper in human serum”,
Analyst, 138, 6097-6105 (2013).
[DOI: 10.1039/C3AN01107K] Open Access

Review

冨田 峻介, 吉本 敬太郎 “交差反応型センサアレイを用いるクルードなタンパク質溶液の評価”
生物工学会誌, 93, 285-288 (2015).
[PDF]

2010-2012

Yuan, X., Fabregat, D., Yoshimoto, K., Nagasaki, Y.*, “Development of a high-performance immunolatex based on “soft landing” antibody immobilization mechanism”,
Colloids and Surfaces B: Biointerfaces, 99, 45-52 (2012).

Ikeda, Y., Jomura, T., Horiuchi, U., Saeki, J., Yoshimoto, K., Ikeya, T., Nagasaki, Y.*, “Long-term survival and functional maintenance of hepatocytes by using a microfabricated cellarray”,
Colloids and Surfaces B: Biointerfaces, 97, 97-100 (2012).

Yuan, X., Fabregat, D., Yoshimoto, K., Nagasaki, Y*. “High PEGylation Efficiency of Pentaethylenehexamine-end Poly(ethyleneglycol) (mPEG-N6) for Active-ester Surface”,
Colloids and Surfaces B: Biointerfaces, 92,25-29 (2012).

Saito, S.*, Nakano, Y., Hikichi, A., Suzuki, R., Yoshimoto, K., Maeda, M., Aoyama, M., Shibukawa, M., “Ultrasensitive CE for heavy metal ions using the variations in the chemical structures formed from new octadentate fluorescent probes and cationic polymers”
Analyst, 136(13),2697-2705 (2011).

Kubota, M., Yoshimoto, K., Yuan, X., Nagasaki, Y.*, “Improvement of the thermal stability of streptavidin immobilized on magnetic beads by the construction of a mixed-poly(ethylene glycol) tethered-chain Layer”
Polymer Journal, 43, 493-496 (2011).

Yoshimoto, K., Nishio, M., Sugasawa, H., and Nagasaki, Y.*, “Direct Observation of Adsorption-Induced Inactivation of Antibody Fragments Surrounded by Mixed-PEG Layer on a Gold Surface”,
Journal of the American Chemical Society, 132(23), 7982-7989 (2010).

Ganguli, S., Yoshimoto, K., Tomita, S., Sakuma, H., Matsuoka, T., Shiraki, K., and Nagasaki, Y.*, “Improving the Heat Resistance of Ribonuclease A by the Addition of Poly(N,N-diethylaminoethyl methacrylate)-graft-poly(ethylene glycol) (PEAMA-g-PEG)”,
Macromolecular Bioscience, 10, 853-859 (2010).